產品資料
  首頁 >>> 產品目錄 >>> 細胞因子 >>> 蛋白酶

重組牛EK, His

產品名稱: 重組牛EK, His
產品型號: PP1002148-1000IU
產品展商: PERFEMIKER
產品價格: 3040.00 元
會員價格: 0.00 元
產品文檔: 無相關文檔
我要買:
產品已經成功添加到購物車
你添加了1件產品 查看購物車

簡單介紹

名稱:重組牛EK, His 貨號:PP1002148 規格:1000IU


重組牛EK, His  的詳細介紹

名稱:重組牛EK, His

Synonyms:Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15

Source:Escherichia coli.

Molecular Weight:Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 241 amino acids,with 6 × His at C-terminus.

Quantity:100IU/250IU/1000IU

Unit Definition:One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 25 °C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.

Physical Appearance:Sterile liquid.

Formulation:50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol.

Endotoxin:Less than 1 EU/μg of rBoEKL, His as determined by LAL method.

Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 6 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after opening.

Reference:1. Yuan LDandHua ZC. 2002. Protein Expr Purif, 25: 300-4.
2. Peng L, Zhong X, Ou J, et al. 2004. J Biotechnol, 108: 185-92.
3. Light AandJanska H. 1991. J Protein Chem, 10: 475-80.
4. Kubitzki T, Minor D, Mackfeld U, et al. 2009. Biotechnol J, 4: 1610-8.

Background:Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications. rBoEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

 

伊人久久精品无码二区